Eppendorf twin.tec® real-time PCR Plates

Image – twin.tec real-time PCR plate 96 group stacked
Image – twin.tec realtime PCR
Image – Forensic DNA Grade
Image – Forensic DNA Grade
Image – twin.tec real-time PCR plate 96 group stacked
Image – twin.tec realtime PCR
Image – Forensic DNA Grade
Image – Forensic DNA Grade
Eppendorf twin.tec® real-time PCR Plates

White wells increase the reflection of fluorescence in real-time PCR and other fluorescence assays. Increased fluorescence improves the sensitivity of your assays, while also improving reproducibility and homogeneity of your results. More information

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Product Information

Eppendorf twin.tec real-time PCR Plates offer all the advantages of regular twin.tec plates and give you the advantage of white wells for your real-time PCR. The limiting factor in low volume real-time PCR is often the remaining intensity of fluorescence. The white wells of the Eppendorf twin.tec real-time PCR Plates reflect fluorescence much better than clear or frosted wells. Thus, lower levels of fluorescence are still detectable with the same instrument – just by using the right consumables. Additionally, white wells significantly reduce interfering background fluorescence and lead to increased homogeneity of replicates and reproducible results. The rigidity of the polycarbonate frame ensure easy and reliable handling – manually or automated. The polypropylene wells guarantee excellent and fast temperature transfer to the sample.

High intensity of fluorescence improves sensitivity in qPCR. This graph shows you an up to 10-fold higher fluorescence measurement of twin.tec real-time PCR plates than those with frosted wells.

Eppendorf twin.tec® real-time PCR Plates

Eppendorf twin.tec® real-time PCR plates reduce Ct-values more effectively than other tested plates. On average, Ct-values occur 0.8 cycles earlier (compared to clear wells), indicating better sensitivity.

Eppendorf twin.tec® real-time PCR Plates

White wells improve homogeneity of results over the block. In this graph you can see the reduced Standard Deviation over the block compared to other plates.

Eppendorf twin.tec® real-time PCR Plates

Features

  • White wells for better reflection
  • High mechanical stability
  • Effective sealing by raised well rims
  • Skirted and semi-skirted frames
  • Optimal heat transfer due to reduced wall thickness
  • Autoclavable (121 °C, 20 min)
  • Available with barcode (upon request)

Applications

  • Real-time PCR
  • Melting Curve Analysis
 Eppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR PlatesEppendorf twin.tec® 96 real-time PCR Plates
Bottom shapeV bottomV bottomV bottomV bottomV bottomV bottomV bottomV bottom
Frame Designskirtedsemi-skirtedskirtedskirtedsemi-skirtedsemi-skirtedunskirted, low profileunskirted, low profile
Materialpolypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)polypropylene (wells), polycarbonate (skirt)
Number of wells96 wells96 wells96 wells96 wells96 wells96 wells96 wells96 wells
OptiTrack® frame colorwhitewhitebluewhitebluewhitewhiteblue
Packing10 plates, individually wrapped10 plates, individually wrapped25 plates25 plates25 plates25 plates20 plates20 plates
Purity grade(s)Forensic DNA GradeForensic DNA GradePCR cleanPCR cleanPCR cleanPCR cleanPCR cleanPCR clean
Volume150 µL250 µL150 µL150 µL250 µL250 µL150 µL150 µL
Well colorwhitewhitewhitewhitewhitewhitewhitewhite
Eppendorf twin.tec® real-time PCR Plates
Video: Eppendorf twin.tec® real-time PCR Plates – Video – Eppendorf Consumables - behind the scenes!

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