BioBLU® c Single-Use Vessels

Image – BioBLU 3c straight
Image – BioBLU 5p with medium, front view
Image – BioBLU c Single-Use Vessels Group 0.3c 1c 3c 10c 50c
Image – BioBLU 3c straight
Image – BioBLU 5p with medium, front view
Image – BioBLU c Single-Use Vessels Group 0.3c 1c 3c 10c 50c
BioBLU® c Single-Use Vessels

Made from monolayer virgin plastics, the Eppendorf BioBLU Single Use Vessels address the widely discussed problems associated with leachables and extractables (L&E), helping to make laboratories more efficient and safe. The rigid wall stirred–tank design mimics traditional vessels and makes scale-up easy. More information

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Product Information

Eppendorf BioBLU Single-Use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design. Single-use vessels eliminate autoclaving, improve turn-around time, simplify validation, and reduce overall costs. The BioBLU portfolio has grown to accommodate users from early research and development through manufacturing. BioBLU c and p vessels cover a wide variety of processes, including shear-sensitive suspension cell cultures and adherent cell line development.

Features

  • Single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 100 mL to 40 L working volume
  • All BioBLU c vessel sizes available with pitched-blade impellers for cell culture applications
  • BioBLU 5p vessels with Eppendorf-exclusive packed-bed impeller, pre-loaded with Fibra-Cel® Disks
  • Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
  • Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
  • Vessels are assembled with sparger, overlay, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
  • For use with Eppendorf benchtop bioreactor systems
  • Adaptor kits for legacy bioreactor controllers available
  • Technical and material documentation available to support process validation activities

Applications

  • Insect, mammalian, and human suspension cell lines
  • Stem cells
  • Adherent cells
 BioBLU® 5cBioBLU® 14cBioBLU® 0.3cBioBLU® 0.3cBioBLU® 1cBioBLU® 1cBioBLU® 1cBioBLU® 1cBioBLU® 3cBioBLU® 3cBioBLU® 3cBioBLU® 3cBioBLU® 10cBioBLU® 10cBioBLU® 50cBioBLU® 50cBioBLU® 5pBioBLU® 5p
Validatableyesyes
Standard set-upBenchtopBenchtopDASboxDASboxBioblock/BenchtopBioblock/BenchtopBioblock/BenchtopBioblock/BenchtopBenchtopBenchtopBenchtopBenchtopBenchtopBenchtopBenchtopBenchtopBenchtopBenchtop
Bioreactors                  
SterilizationPre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)
Working volume (total)1.25 – 3.75 L (5 L)3.5 – 10.5 L (14 L)100 – 250 mL (380 mL)100 – 250 mL (380 mL)320 mL – 1.25 L (1.8 L)1320 mL – 1.25 L (1.8 L)1320 mL – 1.25 L (1.8 L)1320 mL – 1.25 L (1.8 L)11.25 – 3.75 L (5 L)1.25 – 3.75 L (5 L)1.25 – 3.75 L (5 L)1.25 – 3.75 L (5 L)3.3 – 10 L (13.3 L)3.3 – 10 L (13.3 L)18 – 40 L (50 L)18 – 40 L (50 L)3.75 L (5 L)3.75 L (5 L)
Material vessel3PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PC (USP Class VI)PC (USP Class VI)PC (USP Class VI)PC (USP Class VI)PC (USP Class VI)PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)
Material tubingSilicone, C-FlexSilicone, C-FlexSilicone, C-Flex2Silicone, C-Flex2Silicone, C-Flex2Silicone, C-Flex2Silicone, C-Flex2Silicone, C-Flex2Silicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-Flex
Head plate ports1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 1x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 1x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5 (1x 19 mm to Pg 13.5), 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5 (1x 19 mm to Pg 13.5), 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust
Agitation                  
DriveMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drives
ImpellersPitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePitched-bladePacked-bedPacked-bed
Recommended agitation speed25 – 200 rpm25 – 200 rpm20 – 500 rpm20 – 500 rpm30 – 600 rpm30 – 600 rpm30 – 600 rpm30 – 600 rpm25 – 200 rpm25 – 200 rpm25 – 200 rpm25 – 200 rpm25 – 200 rpm25 – 200 rpm25 – 150 rpm25 – 150 rpm25 – 200 rpm25 – 200 rpm
Gassing                  
Gas supplySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlay
Monitoring and Control                  
Sensor length225 mm355 mm120 mm120 mm220 mm220 mm220 mm220 mm225 mm225 mm225 mm225 mm355 mm355 mm526 mm526 mm120 mm120 mm
Temperature sensorPt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)
Max. operating temperature40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C40 °C
pH sensorOptical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (325 mm)Optical pH or standard glass sensor (120 mm)Optical pH or standard glass sensor (120 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (325 mm)Optical pH or standard glass sensor (325 mm)Optical pH or standard glass sensor (625 mm)Optical pH or standard glass sensor (625 mm)Optical pH or standard glass sensor (120 mm)Optical pH or standard glass sensor (120 mm)
DO SensorPolarographic DO - 12/225 mmPolarographic DO - 12/355 mmPolarographic DO - 4.7/118 mmPolarographic DO - 4.7/118 mmPolarographic DO - 4.7/229 mmPolarographic DO - 4.7/229 mmPolarographic DO - 4.7/229 mmPolarographic DO - 4.7/229 mmPolarographic or optical DO - 12/225 mmPolarographic or optical DO - 12/225 mmPolarographic or optical DO - 12/225 mmPolarographic or optical DO - 12/225 mmPolarographic or optical DO - 12/355 mmPolarographic or optical DO - 12/355 mmPolarographic DO - 12/526 mmPolarographic DO - 12/526 mmPolarographic DO - 12/120 mmPolarographic DO - 12/120 mm
Exhaust condensationElectric heater band or PeltierElectric heater bandLiquid-free cooling (Peltier)Liquid-free cooling (Peltier)Liquid-free cooling (Peltier)/water-cooledLiquid-free cooling (Peltier)/water-cooledLiquid-free cooling (Peltier)/water-cooledLiquid-free cooling (Peltier)/water-cooledElectric heater band or PeltierElectric heater band or PeltierElectric heater band or PeltierElectric heater band or PeltierElectric heater bandElectric heater bandElectric heater bandElectric heater bandElectric heater band or PeltierElectric heater band or Peltier
1425 mL minimal working volume when used with vessel stand and heating blanket
2C-Flex is for optical pH version only.
3PC = Polycarbonate, PS = Polystyrene
BioBLU® c Single-Use Vessels
Video: BioBLU® c Single-Use Vessels – Video – BioBLU Vessel Setup